DSIP
Delta Sleep-Inducing Peptide — 9-amino-acid neuropeptide
DSIP (delta sleep-inducing peptide) is a nine-amino-acid peptide first isolated in the 1970s from the blood of rabbits in which sleep had been induced. It is used in practitioner protocols to try to deepen sleep. Evidence comes from animal studies and small, older human studies with inconsistent results; it is not an approved drug, and how it works is still unclear.
Quick reference
How DSIP is dosed in practice
Protocols use 100–300 mcg of DSIP injected SubQ once a night, 30–60 minutes before bed. Courses usually last 2–4 weeks, then about 2 weeks off.
Many start at 100 mcg and increase only if sleep hasn't changed after several nights. Grogginess the next morning is a sign to go back down.
Where the numbers come from: practitioner convention. There's no modern dosing trial, and this guide doesn't list more than 300 mcg per dose. Some people use DSIP as a nasal spray; there's no data on how much is absorbed that way.
Work out the units to draw with the reconstitution calculator and how long a vial lasts with the vial duration calculator.
How DSIP works
DSIP was isolated in the 1970s from the blood of rabbits in which sleep had been induced; infused into other rabbits, it increased delta-wave (slow-wave) EEG activity. It is a 9-amino-acid peptide (Trp-Ala-Gly-Gly-Asp-Ala-Ser-Gly-Glu).
How DSIP works is still unclear — no specific receptor has been identified. Animal studies suggest effects on sleep regulation and on stress-hormone (cortisol) responses, but small human studies gave inconsistent results on sleep.
Why it's in modern protocols: deep (slow-wave) sleep matters for recovery and is when the body's largest growth hormone pulse occurs, so practitioners use DSIP to try to deepen it, sometimes alongside CJC-1295 + Ipamorelin. Whether DSIP reliably does this in people is unproven.
In the DoseCraft framework, DSIP is filed under ATP Shortage (deep sleep and recovery) and Inflammation (stress response) — a framework placement, not a tested outcome.
Reconstitution math
- Remove the flip-off cap from the 5 mg vial.
- Draw 1 mL of bacteriostatic water into a sterile syringe.
- Inject slowly down the vial wall.
- Swirl gently. Do not shake.
- Refrigerate. Stable approximately 30 days.
Dose math
5 mg vial + 1 mL bac water → 5,000 mcg/mL.
On a 100-unit insulin syringe (1 IU = 0.01 mL):
- • 2 IU = 100 mcg
- • 4 IU = 200 mcg
- • 6 IU = 300 mcg — the top of the range
Safety + side-effect profile
DSIP is short-acting, so it doesn't build up with nightly use. There is little modern safety data.
Not established: long-term safety. Avoid with: pregnancy and breastfeeding. Talk to your doctor first if you have depression or another mental-health condition, or take medicines that affect sleep.
Interaction notes: commonly paired with CJC-1295 + Ipamorelin. Use caution with sleeping pills, benzodiazepines, alcohol or high-dose melatonin — combined effects haven't been studied and may add to grogginess.
- • Vivid dreams
- • Morning grogginess at the top of the range
- • Rare injection-site soreness
Frequently asked
What is DSIP?
DSIP (delta sleep-inducing peptide) is a nine-amino-acid peptide first isolated in the 1970s from the blood of rabbits in which sleep had been induced. It's used to try to deepen sleep. Human evidence is limited and inconsistent, and it is not an approved drug.
What is the typical DSIP dose?
100–300 mcg SubQ once a night, 30–60 minutes before bed, for 2–4 week courses followed by about 2 weeks off. Many start at 100 mcg. This is practitioner convention; there's no modern dosing trial.
How do I reconstitute DSIP?
5 mg vial + 1 mL bacteriostatic water → 5,000 mcg/mL. On a 100-unit insulin syringe, 2 IU = 100 mcg, 4 IU = 200 mcg, 6 IU = 300 mcg. Refrigerate after reconstitution; stable approximately 30 days.
Will DSIP make me sleepy during the day?
It isn't expected to: DSIP is short-acting (its half-life is a matter of minutes) and is taken at bedtime. Some people report morning grogginess at the top of the range — a sign to lower the dose.
DSIP vs melatonin — when to use which?
Melatonin mainly shifts the timing of sleep and is well studied, cheap and widely available. DSIP is used to try to deepen sleep, but the human evidence is weak. Some people use both; starting with one at a time makes it easier to tell what's working.
Should I cycle DSIP?
By convention, yes: 2–4 weeks on, then about 2 weeks off. There's no trial evidence on cycling or tolerance.
Is DSIP addictive or habit-forming?
No dependence or withdrawal has been reported, but DSIP hasn't been studied for long-term use.
Can I stack DSIP with CJC-1295 + Ipamorelin?
It's a common practitioner pairing: the idea is that deeper sleep and the GH-releasing peptides work together, since the largest GH pulse comes in early deep sleep. The combination hasn't been tested, and DSIP's effect on deep sleep in people is unproven.
Protocols using DSIP
Outcome-driven stacks with phased dose schedules and cited PMIDs.
Related compounds
Often researched, stacked, or compared with DSIP.
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